Figure 5.

(a) UVPD spectra of tetrameric concanavalin A [M + 21H]²¹⁺ ions acquired at the orthogonal TPX3 detector by the interaction of 193 nm photons with the ion cloud at different locations of the instrument. (b) Spatial distribution of the product ions and unfragmented precursor ions at the TPX3 detector generated by the photon–ion interaction close to the middle of the TOF region (image corresponds to maroon trace in (a)). 21⁺ charged precursor ion cloud dissociates predominantly into fragment ions that are 13⁺ charged monomers and 8⁺ charged trimers, 12+ charged monomers and 9⁺ charged trimers, or 11⁺ charged monomers and 10⁺ charged trimers. A single laser pulse with an energy of 0.5 mJ (measured at the exit of the attenuator) per TOF cycle was used. A hundred TOF measurement frames were added to produce the total ion spectrum. Table S4 and Figures S16 and S26 show the important data acquisition parameters and event sequence used in external UVPD orthogonal TOF mode.