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[Preprint]. 2023 Jun 26:2023.06.23.546087. [Version 1] doi: 10.1101/2023.06.23.546087

Figure 4. Mitochondrial biogenesis defects in PINK1 p.I368N-1# mutant neurons.

Figure 4

A. Analysis of PARIS, PGC-1α and TH protein level in isogenic control and PINK1 p.I368N 1# mutant neurons by immunoblot.

C. Quantification of immunoblot of PARIS normalized to β-actin. N=3 independent experiments.

D. Quantification of immunoblot of PGC-1α normalized to β-actin. N=3 independent experiments.

E. Quantification of immunoblot of TH normalized to β-actin. N=3 independent experiments.

B. Representative transmission electron microscopy images of neurons from isogenic control and PINK1 p.I368N 1# mutant.

C. Quantification of mitochondrial size per cell body in isogenic control and PINK1 p.I368N 1# mutant neurons. N=15–30 TH positive neurons in each group.

D. Quantification of mitochondrial perimeter per cell body in isogenic control and PINK1 p.I368N 1# mutant neurons. N=15–30 TH positive neurons in each group.

F. Relative mitochondrial DNA copy number quantification of ATP6, NUC, ND1, MT, and COX1 in isogenic control and PINK1 p.I368N 1# mutant neurons. N=3 independent experiments.

E. Analysis of CoxIV and CytC protein level in isogenic control and PINK1 p.I368N 1# mutant neurons by immunoblot.

G. Quantification of immunoblot of CoxIV and CytC in isogenic control and PINK1 p.I368N 1# mutant neurons. N=3 independent experiments.