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[Preprint]. 2023 Jun 26:2023.06.23.546087. [Version 1] doi: 10.1101/2023.06.23.546087

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C. Mitochondrial oxygen consumption rate curve generated using Seahorse platform showing the mitochondrial function of isogenic control and PINK1 p.I368N 1# mutant neurons with or without PARIS knockdown.

D. Quantification of basal respiration in isogenic control and PINK1 p.I368N 1# mutant neurons with or without PARIS knockdown. N=3 independent experiments.

E. Quantification of maximal respiration in isogenic control and PINK1 p.I368N 1# mutant neurons with or without PARIS knockdown. N=3 independent experiments.

F. Representative confocal images of puromycin labelling SUnSET assay in isogenic control and PINK1 p.I368N 1# mutant DA neurons with or without PARIS knockdown.

G. Immunofluorescence intensity quantification of Tom20 and puromycin within TH positive neurons with or without PARIS knockdown. N=20 TH positive neurons in each group.

H. Representative confocal images of SNAP-TAG Cox8a labeled mitochondria with TH staining in isogenic control and PINK1 p.I368N 1# mutant neurons with or without PARIS knockdown.

I. Fluorescence intensity quantification of old and new mitochondria labeled by Cox8a in TH positive neurons with or without PARIS knockdown. N=20 TH positive neurons in each group.

J. Relative mitochondrial DNA copy number quantification of ATP6, NUC, ND1, MT, and COX1 from isogenic control and PINK1 p.I368N-1# mutant neurons with or without PARIS knockdown. N=3 independent experiments.

K. Representative transmission electron microscopy images of neurons from isogenic control and PINK1 p.I368N 1# mutant neurons with or without PARIS knockdown. N=15–30 cells in each group.

L. Quantification of mitochondrial size per cell body from isogenic control and PINK1 p.I368N 1# mutant neurons with or without PARIS knockdown. N=15–30 cells in each group.

M. Quantification of mitochondrial perimeter per cell body from isogenic control and PINK1 p.I368N-1# mutant neurons with or without PARIS knockdown.

N. Representative confocal images of immunofluorescence staining of Tom20 and TH from isogenic control and PINK1 p.I368N 1# mutant neurons with or without PARIS knockdown.

O. Quantification of colocalized Tom20 and TH in isogenic control and PINK1 p.I368N 1# mutant neurons with or without PARIS knockdown. N=10 TH positive neurons in each group.