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[Preprint]. 2023 Jun 30:2023.06.30.547126. [Version 1] doi: 10.1101/2023.06.30.547126

Figure 1. Firefly luciferase and CG6178 both have promiscuous adenylation activity.

Figure 1.

a) Fluc adenylates d-luciferin, and subsequent reaction with oxygen forms an excited-state oxyluciferin, which emits light. CG6178 is incapable of adenylating d-luciferin and has no luciferase activity. b) CG6178 natively functions as a promiscuous long-chain fatty acyl-CoA synthetase (ACSL), ligating coenzyme A to C8-C20 fatty acids via an adenylated intermediate. Fluc also has ACSL activity. c) Both Fluc and CG6178 have luciferase activity with CycLuc2.