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Enzyme concentration and incubation times evaluated for the protoplast isolation from mesophyll in Ricinus communis
| Treatment | Enzymatic concentration (% w/v) | Incubation time (h) |
|---|---|---|
| T1 | Cellulase 1% | 8, 10, 12, 14, 16, 18 |
| Pectinase 1% | ||
| Hemicellulase 0.15% | ||
| T2 | Cellulase 2% | 8, 10, 12, 14, 16, 18 |
| Pectinase 2% | ||
| Hemicellulase 0.3% | ||
| T3 | Cellulase 1.5% | 8, 10, 12, 14, 16, 18 |
| Peptinase Rhizopus sp. 0.4% | ||
| T4 | Cellulase “Onozuka” R-10 1.6% | 14, 16, 18 |
| Macerozyme R-10 0.8% |
