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. Author manuscript; available in PMC: 2023 Jul 7.
Published in final edited form as: Nat Microbiol. 2022 Aug 18;7(9):1404–1418. doi: 10.1038/s41564-022-01176-y

Extended Data Fig. 5 |. Comparison of cholesterol metabolite and gene expression levels between GF and SPF mice.

Extended Data Fig. 5 |

a-c, Cholesterol sulfate levels were quantified in the a) ceca, b) feces, and c) plasma of GF (n=5) and SPF (n=5) and found to be higher in GF mice across these tissues. Data are shown as mean ± SEM with Welch’s two-tailed t-test.

d-f, Cholesterol levels were quantified in the d) ceca, e) feces, and f) plasma of the same mice by GCMS and found to not be significantly different. Data are shown as mean ± SEM with Welch’s two-tailed t-test.

g-i, Expression of host Sult2B1b was measured by quantitative PCR in the g) liver, h) ileum, and i) colon of GF mice (n=5) compared to SPF mice (n=5) and found to have significant differences in both the liver and colon, data are normalized to L32 and shown as mean ± SEM with Welch’s two-tailed t-test.

j-l, Expression of host steroid sulfatase (sts) was measured by quantitative PCR in the j) liver, k) ileum, and l) colon of GF mice (n=5) compared to SPF mice (n=5) and found to be significantly different in the ileum, data are normalized to L32 and shown as mean ± SEM with Welch’s two-tailed t-test..