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. Author manuscript; available in PMC: 2023 Jul 7.
Published in final edited form as: Nat Microbiol. 2022 Aug 18;7(9):1404–1418. doi: 10.1038/s41564-022-01176-y

Extended Data Fig. 9 |. Ch-S levels in mouse intestinal contents and blood.

Extended Data Fig. 9 |

a, Ch-S levels in cecal contents of mice colonized with B. thetaiotaomicron WT were significantly higher than those in GF mice or B. thetaiotaomicron KO colonized mice (GF, n=6; B. theta WT, n=6; B. theta KO, n=6), data are presented as mean ± SEM, one-way ANOVA followed by Tukey’s multiple comparisons.

b, Ch-S levels in fecal contents of mice colonized with B. thetaiotaomicron WT were significantly higher than those in GF mice or B. thetaiotaomicron KO colonized mice (GF, n=6; B. theta WT, n=6; B. theta KO, n=6, data are presented as mean ± SEM, one-way ANOVA followed by Tukey’s multiple comparisons).

c, Ch-S levels in the plasma of mice colonized with B. thetaiotaomicron WT were not significantly higher than those in GF mice or B. thetaiotaomicron KO colonized mice (GF, n=6; B. theta WT, n=6; B. theta KO, n=6, data are presented as mean ± SEM, one-way ANOVA followed by Tukey’s multiple comparisons).

d, No significant differences in the expression of the host gene sult2b1b were observed in the liver of B. theta WT or Δsult-colonized mice compared to GF mice as measured by quantitative PCR (n=6 mice per group), data are normalized to L32 and shown as mean ± SEM with one-way ANOVA followed by Tukey’s multiple comparisons, (n.s. = not significant).

e, Expression of host gene sult2b1b was found to be significantly upregulated in the ileum of GF mice compared to mice colonized with B. theta WT or Δsult as measured by quantitative PCR (n=6 mice per group), data are normalized to L32 and shown as mean ± SEM with one-way ANOVA followed by Tukey’s multiple comparisons.

f, No significant differences in the expression of the host gene sult2b1b were observed in the colon of B. theta WT or Δsult-colonized mice compared to GF mice as measured by quantitative PCR (n=6 mice per group), data are normalized to L32 and shown as mean ± SEM with one-way ANOVA followed by Tukey’s multiple comparisons, (n.s. = not significant).

g-i, No significant differences in the expression of the host gene steryl sulfatase (sts) were observed in the g) liver, h) ileum, or i) colon of B. theta WT or Δsult-colonized mice compared to GF mice as measured by quantitative PCR (n=6 mice per group), data are normalized to L32 and shown as mean ± SEM with one-way ANOVA followed by Tukey’s multiple comparisons, (n.s. = not significant).