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. 2023 Jul 5;79(Pt 7):180–192. doi: 10.1107/S2053230X23005381

Figure 2.

Figure 2

SmTetX causes changes in the UV–Vis spectrum of oxytetracycline (OTC) and NADPH. The initial concentrations of the reagents, which are listed in each panel, were [OTC] = 0.5 mM, [NADPH] = 0.5 mM and [enzyme] = 0.1 µM. The individual enzymatic assays were performed in parallel in 100 mM TAPS pH 8.5. (a–f) Absorbance scans are plotted at 1 min intervals over a time course of 10 min, represented by a rainbow colour gradient [from red through yellow to blue as shown in (a)]. Background absorbance of the buffer was subtracted. (g–l) Monitoring of the decrease in absorbance of OTC (400 nm) and NADPH (340 nm); standard errors of the mean from measurements in triplicate are shown.