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. 2023 Jun 22;14:1220165. doi: 10.3389/fimmu.2023.1220165

Figure 2.

Figure 2

The combination of THD and C. butyricum reduced CINV in CRC mice. (A) The kaolin consumption of mice in different treatment groups (n=3). (B) Fos protein in brain by IHC staining. (C) Fos positive cells were semiquantitatively assessed (n=3). (D) 5-HT of brain in different treatment groups (n=3). (E) the mRNA levels of Tac1 in brain (n=3). (F) 5-HT of colon in different treatment groups (n=3). (G) the mRNA levels of Tac1 in colon (n=3). (H) 5-HT3R and NK1R protein in brain by IHC staining. (I, J) 5-HT3R (I) and NK1R (J) positive cells were semiquantitatively assessed (n=3). Significance determined using one-way ANOVA with Tukey’s multiple comparison test and expressed as mean ± SD, *P < 0.05, **P < 0.01.