Extended Data Table 2 |.
Cryo-EM data collection, refinement and validation statistics
| C5-guano-μOR-Gi-scFvl6 (EMDB-26314) (PDB 7U2L) |
C6-guano-μOR-Gi (EMDB-26313) (PDB 7U2K) |
|
|---|---|---|
| Data collection and processing | ||
|
| ||
| Magnification | 130,000 | 130,000 |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e–/Å2) | 67 | 68.5 |
| Defocus range (μm) | 0.7-2 | 0.7-2 |
| Pixel size (Å) | 1.06 | 0.8676 |
| Symmetry imposed | C1 | C1 |
| Initial particle images (no.) | 2,037,265 | 994,998 |
| Final particle images (no.) | 164,647 | 232,146 |
| Map resolution (Å) | 3.2 | 3.3 |
| FSC threshold | 0.143 | 0.143 |
| Map resolution range (Å) | 3.2-4.9 | 3.2-4.6 |
| Refinement | ||
| Initial model used (PDB code) | 6DDE | 6DDE |
| Model resolution (Å) | 3.2 | 3.2 |
| FSC threshold | 0.143 | 0.143 |
| Model resolution range (Å) | 2.9-3.4 | 2.8-3.4 |
| Map sharpening B factor (Å2) | −161.76 | −103.46 |
| Model composition | ||
| Non-hydrogen atoms | 8739 | 6759 |
| Protein residues | 1122 | 889 |
| Ligands | 1 | 1 |
| B factors (Å2) | ||
| Protein | 60.76 | 92.70 |
| Ligand | 78.28 | 125.85 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.004 | 0.009 |
| Bond angles (°) | 0.566 | 0.768 |
| Validation | ||
| MolProbity score | 1.72 | 1.78 |
| Clashscore | 8.03 | 7.07 |
| Poor rotamers (%) | 0 | 0 |
| Ramachandran plot | ||
| Favored (%) | 95.93 | 94.18 |
| Allowed (%) | 4.07 | 5.82 |
| Disallowed (%) | 0 | 0 |
Statistics of the cryo-EM maps and coordinates.