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. 2023 Jul 3;14:20417314231180050. doi: 10.1177/20417314231180050

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© The Author(s) 2023

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 11. — Histological staining at 4 and 8 weeks after a puncture in vivo: (a) representative paraffin section with H&E and Safranine O/fast green staining at 4 weeks, (b) Histological grading was determined at 4 weeks, (c) representative paraffin section with H&E and Safranine O/fast green staining at 8 weeks, (d) Histological grading was determined at 8 weeks. (In H&E staining, the collagen deposits are shown as a darker pink color, while in Safranin O/fast green staining, the collagen deposits are shown as a Sirius red color; *p < 0.05. **p < 0.01. ***p < 0.001, ns indicates no significant difference).