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. 2023 Mar 3;18(10):2301–2306. doi: 10.4103/1673-5374.369121

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Cell culture of MSCs from rat umbilical cord and collection of EVs.

(A) Eight days after cell culture of the Wharton’s Jelly from rat umbilical cords. The fusiform and polygon-shaped cells (MSCs) reached confluency in 8–10 days. Scale bar: 250 μm. (B) MSC-EVs under a transmission electron microscope. The red arrows indicate MSC-EVs. Scale bar: 500 nm. (C) Nanoparticle tracking analysis of MSC-EVs. (D) Protein expression of CD90 and CD81 (western blotting). CM: Culture medium (deprived of EVs); EV: extracellular vesicle; MSC: mesenchymal stem cell.