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. 2023 Jul 7;9(27):eadd9984. doi: 10.1126/sciadv.add9984

Fig. 5. Chronic activation of gal-3+ macrophages in dystrophic muscle.

Fig. 5.

(A) The number of gal-3+ macrophages in B10.mdx hindlimb muscle, normalized to muscle mass (grams). n = 6 to 19 per time point. (B and C) Representative histograms and quantitative analysis of the geometric MFI of gal-3 in 4-week-old (B) and 52-week-old (C) WT and mdx muscle macrophages. (D) Picrosirius red staining of WT and mdx quadriceps cryosections from 4- and 52-week-old mice. Scale bar, 100 μm. (E and F) Enumeration of gal-3+ macrophages in the VCP-associated inclusion body myopathy mouse model (E) and in the facioscapulohumeral muscular dystrophy Tamoxifen inducible Cre-DUX4 (TIC-DUX4) mouse model (F). n = 4 to 6, 10-month-old mice (E); n = 5, 10-week-old mice (F). (G and H) Regulation of gal-3+ macrophages frequency (G) and number (H) after injury. n = 7 to 9 per time point (G) and (H). *P < 0.05, **P < 0.01, and ****P < 0.0001 using an unpaired Welch’s t test (B), (C), (E), and (F) or two-way ANOVA with Sidak’s multiple comparisons test, for comparison with 2-week time point (A) or with day 0 (G) and (H). ****P < 0.0001 using Sidak’s multiple comparisons test, for comparison with age-matched controls of 4, 8, 12, and 52 weeks, respectively.