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. 2023 Jun 13;12:RP85760. doi: 10.7554/eLife.85760

Figure 3. DSK receptors are expressed in Goro neurons in the larval VNC.

(A and B) Representative images of CCKLR-17D1-T2A-GAL4 (A) and CCKLR-17D3-T2A-GAL4 (B) expressions in the larval CNS. (C and D) Representative images showing double-labeling of CCKLR-17D1-T2A-GAL4 (C) and CCKLR-17D3-T2A-GAL4 (D) with C4da nociceptors (R38A10-lexA). Arrows and arrowheads indicate cell bodies of C4da nociceptors and es cells, respectively. (E and F) Representative images showing double-labeling of CCKLR-17D1-T2A-GAL4 (E) and CCKLR-17D3-T2A-GAL4 (F) with Goro neurons (arrowheads, R69E06-lexA). Expression patterns were confirmed in multiple samples (n=7 and 5). All scale bars represent 20 µm.

Figure 3.

Figure 3—figure supplement 1. Expression patterns of DSK receptors in larval glial cells, peripheral tissue, and nociceptive interneurons.

Figure 3—figure supplement 1.

(A and B) Representative images showing double-labeling of CCKLR-17D1-T2A-GAL4 and a glial cell marker anti-REPO in the larval brain (A) and the larval VNC (B), representing little expressions of CCKLR-17D1-T2A-GAL4 in glial cells. (C and D) Representative images showing double-labeling of CCKLR-17D3-T2A-GAL4 and a glial cell marker anti-REPO in the larval brain (C) and in the larval VNC (D), representing little expressions of CCKLR-17D3-T2A-GAL4 in glial cells. (E and F) Representative images showing the expressions of CCKLR-17D1-T2A-GAL4 (E) and CCKLR-17D3-T2A-GAL4 (F) with a peripheral neuronal marker anti-HRP in the larval body wall, showing the absence of both GAL4s from peripheral sensory neurons other than es cells. (G and H) Expressions of CCKLR-17D1-T2A-GAL4 (G) and CCKLR-17D3-T2A-GAL4 (H) were negligible in Basin1 to 4 neurons labeled by R72F11-lexA in the larval VNC. (I and J) Negligible expression of CCKLR-17D1-T2A-GAL4 (I) and CCKLR-17D3-T2A-GAL4 (J) found in A08n neurons labeled by R82E12-lexA (arrowheads) in the larval VNC. (K and L) Expressions of CCKLR-17D1-T2A-GAL4 (K) and CCKLR-17D3-T2A-GAL4 (L) were negligible in DnB neurons labeled by R70F01-lexA in the larval VNC. All scale bars represent 20 µm.