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. 2023 Jun 13;12:RP85760. doi: 10.7554/eLife.85760

Figure 5. Goro neurons lacking CCKLR-17D1 show sensitized responses to noxious heat.

(A) Representative still images showing thermal activation of Goro neurons in the controls (top, yw/Y; R69E06-GAL4 UAS-GCaMP6m/+) and CCKLR-17D1Δ1 mutants (bottom, CCKLR-17D1Δ1/Y; R69E06-GAL4 UAS-GCaMP6m/+). See also Videos 1 and 2. (B) Average percent increase of GCaMP6m fluorescence intensity relative to the baseline (ΔF/F0) during heat ramp stimulations in controls (n=15) and CCKLR-17D1Δ1 (n=15). * p<0.05, ** p<0.01 Mann-Whitney’s U-test. (C) Basal GCaMP6m signal levels (F0) in controls (n=15) and CCKLR-17D1Δ1 mutants (n=15). p>0.6 Mann-Whitney’s U-test. (D) Representative stills showing thermal activation of the controls (top, R69E06-GAL4 UAS-GCaMP6m x yv; attp2) and Goro neurons expressing CCKLR-17D1 RNAi (bottom, R69E06-GAL4 UAS-GCaMP6m x yv; JF02644). See also Videos 3 and 4. (E) Average percent increase of GCaMP6m fluorescence intensity relative to the baseline (ΔF/F0) during heat ramp stimulations in controls (n=15) and CCKLR-17D1 RNAi (n=16). * p<0.05 Mann-Whitney’s U-test. (F) Basal GCaMP6m levels (F0) in controls (n=15) and CCKLR-17D1 RNAi (n=16). p>0.6 Mann–Whitney’s U-test. Error bars represent standard error. All box plots show median (middle line) and 25th to 75th percentiles with whiskers indicating the smallest to the largest data points. All scale bars represent 20 µm.

Figure 5—source data 1. Source data used to generate the summary data and graphs shown in Figure 5 and Figure 5—figure supplement 1.

Figure 5.

Figure 5—figure supplement 1. Thermal responsiveness of Goro neurons in CCKLR-17D3Δ1.

Figure 5—figure supplement 1.

(A) Representative still images showing thermal activation of Goro neurons in control animals (top, yw/Y; R69E06-GAL4 UAS-GCaMP6m/+) and CCKLR-17D3Δ1 mutants (bottom, CCKLR-17D3Δ1/Y; R69E06-GAL4 UAS-GCaMP6m/+). See also Videos 5 and 6. Scale bars represent 20 µm. (B) Average percent increase of GCaMP6m fluorescence intensity relative to baseline (ΔF/F0) during heat ramp stimulations in CCKLR-17D3Δ1 experiments. ΔF/F0 is plotted to binned probe temperature (interval = 1 °C). In comparison with controls, Goro neurons of CCKLR-17D3Δ1 exhibited mildly elevated fluorescent increase of GCaMP6m, which reached statistical significance only at 44 °C, 45 °C, and 50 °C. n=14 and 16 for controls and CCKLR-17D3Δ1, respectively. * p<0.05, ** p<0.01 (Mann–Whitney’s U-test). Error bars represent standard errors. (C) Basal GCaMP6m signal levels (F0) did not differ between controls and CCKLR-17D3Δ1 (n=14 and 16). p>0.5 (Mann–Whitney’s U-test). Box plots show median (middle line) and 25th to 75th percentiles with whiskers indicating the smallest to the largest data points.