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. 2000 May 1;28(9):1913–1920. doi: 10.1093/nar/28.9.1913

Figure 4.

Figure 4

YR1m4 is defective in supporting transcription in vitro. In vitro transcription assay was performed with a TFIIB-depleted whole cell extract (12). This extract is unable to support transcription without the addition of recombinant TFIIB protein (lane 2). An aliquot of 2 µl of extract (~80 µg of protein) was used in the reaction. G-less cassette plasmid pJJ470 (100 ng) (28) were used as template. Purified recombinant TFIIB protein (50 ng), either wild type or YR1m4, was supplemented as indicated. Activated transcription was assayed upon the addition of 10 ng of purified GAL4-VP16 fusion protein.