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. 2023 Jul 7;13:10991. doi: 10.1038/s41598-023-37134-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Recovered metagenomic data generated through NAS by both enrichment and depletion strategies span whole genome assemblies of bacterial tick-borne pathogens. Circos plot depticting complete genomes of four bacterial TBPs: B. burgdorferi s.s., A. phagocytophilum, E. muris eauclairensis, and B. miyamotoi. Outermost bands are individual ONT reads mapping to each bacterial genome. Reads > 2 kb in length generated during NAS enrichment (Tick 1–Tick 4) are visualized in orange, and > 2 kb reads generated during NAS depletion (Tick 5–Tick 8) are shown in green. Inner track histograms depict mean coverage for mapped bases across pathogen genomes for individual tick samples calculated over a sliding widow of 5 kb.