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. 2023 Jul 7;13:10991. doi: 10.1038/s41598-023-37134-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of reads mapping against the non-target bacterial I. scapularis endosymbiont, R. buchneri. Total R. buchneri-mapped reads generated over NAS depletion, NAS enrichment, and control sequencing experiments are presented as points by read length over a logarithmic scale. Over both NAS-depletion (green) and NAS-enrichment (orange) sequencing strategies, the read length distribution shows a greater proportion of reads < 1000 bp, indicative of real-time rejection of non-target R. buchneri reads during each sequencing experiment. Conversely, during the control experiment (blue), an increased proportion of reads between 1000–10,000 bp is observed, as all non-target R. buchneri reads present in the library were sequenced fully.