Table 3.
Taxonomic classification results for reads generated during NAS and control experiments.
| Bacterial tick-borne pathogen genome | ||||||||
|---|---|---|---|---|---|---|---|---|
| A. phagocytophilum | B. burgdorferi s.s | B. miyamotoi | E. muris eauclairensis | A. phagocytophilum | B. burgdorferi s.s | B. miyamotoi | E. muris eauclairensis | |
| NAS enrichment | Control | |||||||
| Tick 1 | 0 | 274(2.4) | 0 | 769(2.1) | 1 | 113 | 0 | 364 |
| Tick 2 | 0 | 351(1.8) | 5 | 0 | 0 | 194 | 4 | 1 |
| Tick 3 | 0 | 334(1.8) | 1113(2.1) | 2 | 1 | 190 | 523 | 2 |
| Tick 4 | 238(1.8) | 218(1.7) | 5 | 2 | 136 | 127 | 2 | 1 |
| NAS depletion | ||||||||
| Tick 5 | 153 | 657 | 0 | 0 | ||||
| Tick 6 | 665 | 1457 | 1 | 146 | ||||
| Tick 7 | 4 | 190 | 0 | 0 | ||||
| Tick 8 | 14 | 422 | 1 | 0 | ||||
Numbers presented under each TBP taxonomic group indicate the number of ONT reads successfully identified taxonomy at the species level using the kraken2 metagenomic classification pipeline. Numbers within parentheses denote the fold enrichment achieved in classified reads between ticks sequenced during paired adaptive sampling and control experiments.