Reagent | Final concentration | Amount |
---|---|---|
PBS (1×) | N/A | 97 mL |
FBS (100%) | 2% | 2 mL |
EDTA (0.5 M) | 5 mM | 1 mL |
Total | N/A | 100 mL |
Reagent | Final concentration | Amount |
---|---|---|
PBS (1×) | N/A | 97 mL |
FBS (100%) | 2% | 2 mL |
EDTA (0.5 M) | 5 mM | 1 mL |
Total | N/A | 100 mL |