REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
rLV.EF1ZsGreen1-9	Takara Bio	0037VCT
Biological samples
Human neocortical access tissue/GBM tissue samples	Department of Neurosurgery, Medical Centre, University of Freiburg	N/A
Experimental models: Cell lines
BTSC_#233	Uniklinik Freiburg	Primary Cell Line
Chemicals, peptides, and recombinant proteins
SPRIselect reagent	Beckman Coulter	B23318
Bovine serum albumin (BSA)	Sigma-Aldrich	A3059
4′,6-Diamidino-2-phenylindole (DAPI)	Merck KGaA	32670#5MG
Hibernate™ -A Medium	Gibco	12087586
N-methyl-D-Glucamin (NMDG)	Sigma-Aldrich	M2004
Antibiotic-Antimycotic (100×)	Gibco	15240062
Neurobasal™ Medium	Gibco	11570556
L-Glutamine	Gibco	25030149
B-27 Supplement, serum-free (50×)	Gibco	11530536
MgSO4	Sigma-Aldrich	M3409
HEPES	Sigma-Aldrich	H0887
D-Glucose	Sigma-Aldrich	RNBG7039
Potassium chloride (KCl)	Merck KGaA	1.04936.0500
Potassium dihydrogen phosphate (KH2PO4)	Merck KGaA	1.04873.0250
Sodium chloride (NaCl)	VWR	27810.295
Disodium hydrogen phosphate dihydrate (Na2HPO4)	VWR	28029.292
4% Paraformaldehyde solution	Thermo Fisher Scientific	J61899.AK
O.C.T. Tissuetek	Sakura	SA62550-01/-12
Incidin™ Plus	Ecolab	3011520
Fetal bovine serum (FBS)	PAN Biotech	1502
Ethylenediaminetetraacetic acid (EDTA)	Carl Roth	8043.1
Critical commercial assays
KAPA SYBR FAST qPCR Master Mix	Roche	KK4600
Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1	10× Genomics	PN-1000121
Chromium Next GEM Chip G Single Cell Kit	10× Genomics	PN-1000120
Single Index Kit T Set A	10× Genomics	PN-1000213
Fragment Analyzer 5200 HS NGS Kit	Agilent	DNF-474
Qubit 1× dsDNA HS Kit	Thermo Fisher Scientific	Q33231
NextSeq 500/550 High Output Kit v2.5 (150 cycles)	Illumina	20024907
NextSeq 500/550 High Output kit v2.5 (75 cycles)	Illumina	20024906
Neural Tissue Dissociation Kit (T)	Miltenyi Biotec	130-093-231
Software and algorithms
R/R Studio software	Posit	https://posit.co/downloads/
Cell Ranger analysis pipeline	10× Genomics	https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/installation
Cranial Map Neuro Navigation Cart 2	Stryker Corporation	N/A
Other
Leica VT1200 semi-automatic vibratome	Leica	14912000001
Millipore insert	Merck KGaA	PIHP03050
6-well plate	Greiner BIO-ONE	657160
Cryostar™ NX70 cryostat	Epredia	15380755
NIO laser imaging system	Invenio Imaging	01-0224
Hamilton micro syringe	Hamilton	80330
EVOS M7000 microscope	Thermo Fisher Scientific	AMF7000
EVOS onstage incubator	Thermo Fisher Scientific	AMC1000
C-Tube	Miltenyi Biotech	130-093-237
Chromium controller	10× Genomics	N/A
Qubit™ 4 Fluorometer	Thermo Fisher Scientific	Q33238
5200 Fragment Analyzer	Agilent	M5310AA
BD FACSAria™ Fusion	BD Biosciences	N/A
BD FACSAria™ III	BD Biosciences	N/A
MoFlo Astrios EQ	Beckman Coulter	N/A