Fig. 3.

Tau oligomers and tau fibrils both spread to interconnected distal brain regions. a Representative images of phosphorylated AT8 tau pathology in the overlying isocortex (upper panel) and entorhinal cortex (lower panel) of PS19 mice 3 months after injection. Scale bar = 50 µm. b, c Quantification of the number of AT8-positive cells in the cortical areas overlying the dorsal hippocampus and in the perirhinal/entorhinal cortices, respectively, in PS19 mice 3 months after injection. The overlying isocortex comprises the retrosplenial, motor and somatosensory cortices. d, e Quantification of the number of pS422-positive cells in the cortical areas overlying the dorsal hippocampus and in the perirhinal/entorhinal cortices, respectively, in PS19 mice 3 months after injection. f, g Quantification of the number of AT100-positive cells in the cortical areas overlying the dorsal hippocampus and in the perirhinal/entorhinal cortices, respectively, in PS19 mice 3 months after injection. Data represented as mean ± SEM, Kruskal–Wallis, Dunn’s multiple comparison, *p < 0.05, **p < 0.01