Table 1.
Validation of an open-canal automatized d-lactate method.
| Criteria | Quality indicator | Result |
|---|---|---|
| Measurement range | ||
| Limit of Detection (LoD) | Blank test | 0.02 µmol.L−1 |
| Limit of Quantification (LoQ) | <10% relative standard deviation (RSD) | 40 µmol.L−1 |
| Superior limit of linearity | Need for dilution at high-concentration | >15 mmol L−1 |
| Analytical performances | ||
| Within-run imprecision | Coefficient of Variation (CV%) | IQClow: 1.98% |
| IQCmedium: 1.24% | ||
| IQChigh: 0.87% | ||
| Between-run imprecision | IQCmedium: 2.21% | |
| Robustness | CV ratio | 1.63 |
| Interferences | ||
| Hemolysis | ±10% maximal deviation | 300 mg dL−1 |
| Icterus | 103 µmol.L−1 | |
| Lipemia | L index of 47 (highly opalescent) | |
| l-Lactate Dehydrogenase | 1000 IU.L−1 | |
| Storage time | 72 hours in plasma at 4 °C | |