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. 2023 Jun 8;26(7):107070. doi: 10.1016/j.isci.2023.107070

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© 2023 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Single cell transcriptomics of 5 dpf zebrafish ENS

(A) UMAP of 1369 ENS cells, containing eleven different clusters.

(B) Dot plot showing expression of genes highly differentially expressed between clusters.

(C) Maximum projection from FISH recordings of 5 dpf tg(phox2bb:GFP) larvae stained for mmp17b (magenta). Scale bar represents 20 μm.

(D) Single plane detailed images of FISH of 5 dpf tg(phox2bb:GFP) larvae showing co-localization of dapi (blue), mmp17b (magenta) and phox2bb (green) in the intestine. Arrows highlight cells of interest showing co-localization. Scale bar represents 20 μm.