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. 2023 Jun 8;26(7):107070. doi: 10.1016/j.isci.2023.107070

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© 2023 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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The 5 dpf intestine contains four clusters of differentiated neurons including a cluster of elavl3+; phox2bb-enteric neurons showing excitatory and inhibitory gene expression

(A) Featureplots of genes defining four clusters of differentiated neurons.

(B) Featureplots highlighting the presence of a cluster of cells expressing elavl3, slc17a6b and gad1b, but lacking expression of phox2bb (phox2bb-differentiated neurons, depicted by the circle).

(C) Maximum projections of live imaging recordings from 7 dpf tg(gad1b:GFP); tg(vglut2a-dsRed) larval intestine shows overlap between the two reporters (arrows) with one gad1b+ cell that is vlgut2- (arrowhead). Scale bar represents 40 μm.

(D) Maximum projections of live imaging recordings from 7 dpf tg(phox2bb:GFP); tg(vglut2a-dsRed) larval intestine shows no overlap between the two reporters. Scale bar represents 20 μm.