REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
HuC/HuD Neuronal Protein Mouse Monoclonal Antibody	Molecular Probes/Thermofisher	Cat#A-21271; RRID:AB_221448
Connexin-43 (E7N2R) XP Rabbit mAb	Cell Signaling Techn	Cat#83649
Chemicals, peptides, and recombinant proteins
Anti Mouse Cy3	Jackson	Cat#715-165-150; RRID:AB_2340813
Monovalent AffiniPure Fab Fragment Cy3	Jackson	Cat#711-167-003; RRID:AB_2340606
Donkey anti-rabbit Alexa Fluor 488	Thermofisher	Cat#A-21206; RRID:AB_2535792
Goat- anti-mouse Alexa Fluor 568	Thermofisher	Cat#A-11004; RRID:AB_2534072
DAPI	Sigma-Aldrich	Cat#D-9542
Ultrapure Low melting	ThermoFisher	Cat#16520050
MS-222, Tricaine	Supelco	#A5040-250G
Papain	Sigma	Cat#P4762
HBSS with CaCl2 and MgCl2	Gibco	Cat#14025050
L-Cystein	Sigma	Cat#C7352
Tween 20	Sigma	Cat#P7949-500
Triton X-100	Sigma	Cat#T8787
BSA	Sigma-Aldrich	Cat#A3294
Horse serum	Invitrogen	Cat#16060-122
Glycerol	Riedel-de Haën	Cat#49770-1L-D
Trypan blue	Sigma	Cat#T8154
Critical commercial assays
RNAscope Multiplex Fluorescence Reagent Kit v2 Assay	ACDbio	Cat#323100
10X Chromium Single Cell 3′ Kit	10X Genomics	Cat# 120237
Mmp17b probe	ACDbio	Custom
Opal 570 dye	Akoya	SKU FP1488001KT
Deposited data
Raw single-cell RNA-seq data	This study	GEO: GSE225510
Processed single-cell RNA-seq data	This study	GEO: GSE225510
Experimental models: Organisms/strains
Zebrafish tg(phox2bb:GFP)	Nechiporuk et al. (2007)	ZDB-TGCONSTRCT-070522-1
Zebrafish tg(8.3phox2bb:kaede)	Harrison et al. (2014)	ZDB-TGCONSTRCT-150305-1
Zebrafish tg(her4:GFP)	Sang-Yeob et al.(2007)	ZDB-TGCONSTRCT-070612-3
Zebrafish tg(gfap:GFP)	Raymond et al. (2006)	ZDB-TGCONSTRCT-070117-154
Zebrafish tg(vglut2:loxp-dsRed-loxP-GFP)	Miyasaka et al. (2009)	ZDB-TGCONSTRCT-070117-143
Zebrafish tg(gad1b:GFP)	Satou et al. (2013)	ZDB-TGCONSTRCT-131127-6
Software and algorithms
Cell Ranger v3.0.2	10X Genomics	N/A
Fiji	ImageJ	National Center for Microscopy and Imaging Research: ImageJ Mosaic Plug-ins, RRID:SCR_001935
Leica	LASX	Leica Application Suite X, RRID:SCR_013673
Adobe illustrator	Adobe Inc.	N/A
Seurat v3	Stuart et al. (2019)	https://satijalab.org/seurat/
Monocle 3	Trapnell et al. (2014)	https://cole-trapnell-lab.github.io/monocle3/
R version 4.0.3	R Core Team (2022)	https://www.R-project.org/
RStudio 1.2.5033	RStudio Team (2020)	http://www.rstudio.com/
Prism 5	Graphpad	GraphPad Prism, RRID:SCR_002798