Figure 1.

NK cells exert potent anti-tumor activity against melanoma cell lines

(A) Terminally differentiated NK cells were phenotypically characterized at day 35 for NK cell receptors, and percentages and gMFI are shown.

(B and C) Flowcytometry-based cytotoxicity results against 9 melanoma cell lines of a (B) 5h co-culture and (C) 20h co-culture assay, E:T ratio of 1:1. The order of the cell lines in the graphs is arranged from high to low susceptibility for NK cells in the 5-h co-culture assay.

(D) Percentage of degranulating cells after 5h co-culture with melanoma cells. Each data point represents an individual donor and data are shown as mean ± SEM (n = 9, for C n = 8, divided among 3 independent experiments).

(E) The corresponding heatmap for cytotoxicity 5h and degranulation 5h of the individual donors. The individual donors are arranged in increasing cytotoxic capacity based on the geometric mean (gMean) of % cytotoxicity of each individual donor against all cell lines.