Figure 5.

Effect on cytotoxicity of melanoma cell lines on blocking of various receptors

NK cells were pre-incubated with different combinations of blocking mAbs and seeded in co-culture with A375 in a 1:1 E:T ratio for 24h. Representative graphs of A375 cytotoxicity in co-culture with donor #7 show the mean of technical triplicates after blocking:

(A) DNAM-1, NKG2D, or TRAIL.

(B) Combinations of DNAM-1, NKG2D and TRAIL.

(C) NKp30, NKp44 or NKp46.

(D) Combinations of NKp30, NKp44 or NKp46 with TRAIL.

(E) Combinations of NKp30, NKp44 and NKp46.

(F) Combinations of two out of NKp30, NKp44 or NKp46 with TRAIL or all four combined. All blocking mAbs were used at 10 μg/mL. Concentration of isotype ctrl Ab is matched to the concentration of four added blocking mAbs.

(G) Heatmap of results of blocking studies for 2 donors against 3 melanoma cell lines. The percentage of cytotoxicity relative to isotype ctrl for each blocking combination is shown for MEL-BRO, SK-MEL-28 and A375 for donor #5 and #7. Percentage was calculated as (AUC condition/AUC isotype ctrl)∗100. AUC = area under the curve.