REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-CD45 KO (J.33)	Beckman Coulter	Cat# B36294; RRID: AB_2833027
anti-CD56 APCA750 (N901)	Beckman Coulter	Cat# B46024
anti-NKG2D PE (ON72)	Beckman Coulter	Cat# A08934; RRID: AB_2801262
anti-NKp44 PE (Z231)	Beckman Coulter	Cat# A66903
anti-NKG2A PE (Z199)	Beckman Coulter	Cat# IM3291U; RRID: AB_10643228
anti- KIR2DL1 KIR2DS1 PE (EB6.B)	Beckman Coulter	Cat# A09778; RRID: AB_2801261
anti-DNAM-1 PE (REA1040)	Miltenyi Biotec B.V.	Cat# 130-117-489; RRID: AB_2727959
anti-NKp30 BV605 (p30-15)	BD Biosciences	Cat# IM3709
anti-NKp46 BV786 (9E2)	BD Biosciences	Cat# 563329; RRID: AB_2738139
anti-FASL BV605 (NOK-1)	BD Biosciences	Cat# 306418
anti-TRAIL BV786 (RIK-2)	BD Biosciences	Cat# 743723; RRID: AB_2741699
anti-MICA/B APC (6D4)	Biolegend	Cat# 320908; RRID: AB_493195
anti-nectin-2 PECy7 (TX31)	Biolegend	Cat# 337414; RRID: AB_2565732
anti-PVR PerCP/Cy5.5 (SKII.4)	Biolegend	Cat# 337612; RRID: AB_2565536
anti-PCNA AF488 (PC10)	Biolegend	Cat# 307909; RRID: AB_2160546
anti-FAS AF488 (DX2),	Biolegend	Cat# 305616; RRID: AB_528891
anti-TRAILR1 APC (DJR1)	Biolegend	Cat# 307208; RRID: AB_2256112
and anti-TRAILR2 PE (DJR2-4)	Biolegend	Cat# 307406; RRID: AB_2204667
anti-ULBP- 2/5/6 BUV661 (165903)	BD Biosciences	Cat# 750011; RRID: AB_2874231
anti-ULBP-1 AF488 (170818)	R&D systems	Cat# FAB1380G
anti-ULBP-3 PE (166510)	R&D systems	Cat# FAB1517P; RRID: AB_10719122
anti-B7-H6 AF647 (1A5)	BD Biosciences	Cat# 566733; RRID: AB_2869835
anti-TRAILR3 BV421 (B-D44)	BD Biosciences	Cat# 744764; RRID: AB_2742464
anti-TRAILR4 FITC (TRAIL-R4-01)	Thermofisher	Cat# 12392183
anti-CD107a PE (H4A3)	Biolegend	Cat# 328608; RRID: AB_1186040
anti-IFNγ PE (B27)	Biolegend	Cat# 506507; RRID: AB_315440
anti-TNF AF488 (MAb11)	Biolegend	Cat# 502915; RRID: AB_493121
anti-CD56 BV421 (NCAM16.2)	BD Biosciences	Cat# 562751; RRID: AB_2732054
anti-Perforin PerCP-Cy5.5 (B-D48)	Biolegend	Cat# 353314; RRID: AB_2571971
anti-Granzyme B AF647 (GB11)	Biolegend	Cat# 515406; RRID: AB_2566333
mouse IgG1 isotype ctrl PE (MOPC-21)	Biolegend	Cat# 400114; RRID: AB_2847829
mouse IgG1 isotype ctrl AF488 (MOPC-21)	Biolegend	Cat# 400129; RRID: AB_2890263
mouse IgG1 isotype ctrl PerCP-Cy5.5 (MOPC-21)	Biolegend	Cat# 400149; RRID: AB_893680
mouse IgG1 isotype ctrl AF647 (MOPC-21)	Biolegend	Cat# 400130; RRID: AB_2800436
anti-NKG2D (1D11)	Biolegend	Cat# 320814; RRID: AB_2561488
anti-TRAIL (RIK-2),	Biolegend	Cat# 308214; RRID: AB_2814155
anti-NKp30 (P30-15),	Biolegend	Cat# 325224; RRID: AB_2814183
anti-NKp44 (P44-8),	Biolegend	Cat# 325122; RRID: AB_2819955
anti-NKp46 (9E2)	Biolegend	Cat# 331948; RRID: AB_2814217
anti-DNAM-1 (DX11)	BD Biosciences	Cat# 559786; RRID: AB_397327
Anti-FASL (NOK-1)	Biolegend	Cat# 306416; RRID: AB_2810459
Mouse IgG1, kappa (MOPC-21)	Biolegend	Cat# 400166; RRID: AB_11146992
Bacterial and virus strains
IncuCyte NucLight Red (NLR) Lentivirus	Essen Biosciences	Cat# 4476
Biological samples
Fresh umbilical cord blood	Anthony Nolan cord blood bank	NA
Fresh umbilical cord blood	NHS Blood and Transplant	Cat# M185
Human serum	Sanquin	Cat#B0618
Chemicals, peptides, and recombinant proteins
7-aminoactinomycin D	Sigma	Cat# A9400-1MG
Ficoll Paque Plus	GE Healthcare	Cat# GE17-1440-03
pacific blue succinimidyl ester (PBSE)	Thermofisher	Cat#P-10163
PMA	Merck Life Sciences	Cat#P1585-1MG
Ionomycin	Merck Life Sciences	Cat#I9657
Live/dead fixable aqua	Thermofisher	Cat#L34957
rhTRAIL	Biolegend	Cat#752906
Critical commercial assays
CD34+ microbead kit	Miltenyi Biotech B.V.	Cat#130-046-702
cytofix/cytoperm kit	BD Biosciences	Cat# 554715
CD8/NK LEGENDplex™	Biolegend	Cat# 740267
Incucyte® Caspase-3/7 Dye	Sartorius	Cat# 4440
Cell Meter™ Multiplexing Caspase 3/7, 8 and 9 Activity Assay Kit	Bioconnect	Cat# 22820_100tests
Experimental models: Cell lines
A375	ATCC	Cat# CRL-1619; RRID: CVCL_0132
COLO829	ATCC	Cat# CRL-1974; RRID: CVCL_1137
G361	ATCC	Cat# CRL-1424; RRID: CVCL_1220
RPMI-7951	ATCC	Cat# HTB-66; RRID: CVCL_1666
MeWo	ATCC	Cat# HTB-65; RRID: CVCL_0445
SK-MEL-28	CLS	Cat#300337; RRID: CVCL_0526
MEL-JUSO	DSMZ	Cat# ACC 74; RRID: CVCL_1403
MEL-BRO	NA	RRID: CVCL_7036
WM9	NA	RRID: CVCL_6806
Software and algorithms
Graphpad Prism software 9.0	GraphPad Software	RRID: SCR_002798
Kaluza v2.1	Beckman Coulter	RRID: SCR_016182
Cbioportal for Cancer Genomics	Cbioportal for Cancer Genomics	RRID:SCR_014555
TCGA-SKCM	PanCancer Atlas	https://doi.org/10.25504/FAIRsharing.m8wewa
BioRender	BioRender	BioRender.com
Other
DMEM with UltraGlutamine	Lonza/Westburg	Cat# LO BE12-604F/U1
RPMI 1640 with UltraGlutamine	Lonza/Westburg	Cat# LO BE12-702F/U1
EMEM with L-Glutamine	Lonza/Westburg	Cat# LO BE12-611F
McCoy5a with L-Glutamine	Lonza/Westburg	Cat# LO BE12-688F
FBS	Gibco/Thermofisher	Cat# 10500-064
TryplExpress	Thermofisher	Cat#10718463
Glycostem Basal Growth Medium	Fertipro	Cat# GBGM-500
TPO	Cellgenix	Cat# 1017-050
IL-7	Cellgenix	Cat# 1010-050
Flt-3L	Cellgenix	Cat# 1015-050
GM-CSF	Cellgenix	Cat# 1012-050
Neupogen (G-CSF)	Amgen B.V.	NA
Il-6	Cellgenix	Cat#1004-050
SCF	Cellgenix	Cat#1018-050
IL-15	Cellgenix	Cat# 1013-050
Proleukin (IL-2)	Novartis	NA