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. 2023 Jun 21;15(7):evad116. doi: 10.1093/gbe/evad116

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© The Author(s) 2023. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1 — A) The map of the potential distribution of Unio pictorum generated by overlapping the points of recent presence records (obtained from 13) with level 5 polygons of the Hydrobasinslayer. On the top left is shown the U. pictorum specimen used for the WGA. B) Left: GenomeScope2 k-mer (21) distribution displaying the estimated genome size (len), homozygosity (aa), heterozygosity (ab), mean k-mer coverage for heterozygous bases (kcov), read error rate (err), the average rate of read duplications (dup), k-mer size used in the run (k:), and ploidy (p:). Right: Assessment of the U. pictorum genome assembly using the KAT comp tool to compare the PacBio Hi-Fi k-mer content within the genome assembly after running purge_dups. Different colors represent the read k-mer frequency in the assembly.