Table 1.
General Statistics of the Unio pictorum Final Genome Assembly (p_ctg); U. pictorum Alternative Haplotypes Genome Assemblies (hap1 and hap2); Other Published Freshwater Mussels Genome Assemblies
| Hifiasm -s 0.75 purge_dups p_ctg | Hifiasm -s 0.75 hap1 | Hifiasm -s 0.75 hap2 | Megalonaias nervosa | Potamilus streckersoni | Margaritifera margaritifera V1 | Margaritifera margaritifera V2 | Unio delphinus | Venustaconcha ellipsiformis | Hyriopsis cumingii | |
|---|---|---|---|---|---|---|---|---|---|---|
| Total number of sequences | 670 | 3,357 | 2,702 | 96,310 | 2,366 | 105,185 | 1,700 | 1,254 | 371,427 | 77.26 |
| Total length (Gb) | 2.43 | 2.44 | 2.35 | 2.36 | 1.77 | 2.47 | 2.45 | 2.50 | 1.59 | 3.38 |
| N50 length (Mb) | 10.61 | 3.59 | 3.66 | 0.050 | 2.05 | 0.29 | 3.43 | 10.91 | 0.006 | 84.3 |
| L50 | 71 | 181 | 174 | 12,463 | 245 | 2,393 | 207 | 67 | 58,531 | 15 |
| Largest contig (Mb) | 44.85 | 26.90 | 20.62 | 0.58 | 10.78 | 2.51 | 23.80 | 43.58 | 0.31 | 158.3 |
| GC content, % | 34.82 | 34.84 | 2,698 | 35.82 | 33.79 | 35.42 | 35.30 | 35.07 | 34.19 | 36.07 |
| Total BUSCO for the genome assembly (%) | ||||||||||
| # Euk database | S:96.1% D:3.1% F:0.8% | S:92.5% D:2.7% F:2.0% | S:91.4% D:2.7% F:2.0% | S:70.2% D:0.4% F:14.9% | S:97.3% D:0.8% F:0.8% | S:85.8% D:1.0% F:5.9% | S:97.6% D:1.6% F:0.4% | S:96.1% D:2.4% F:1.6% | S:45.5% D:0.4% F:36.9% | S:92.2% D:0.8% F:3.1% |
| # Met database | S:93.7% D:2.6% F:2.4% | S:90.1% D:2.2% F:2.5% | S:90.6% D:2.3% F:2.8% | S:70.1% D:1.4% F:14.5% | S:93.6% D:1.4% F:2.3% | S:83.8% D:1.1% F:4.9% | S:95.5% D:1.4% F:2.0% | S:94.4% D:2.1% F:2.3% | S:52.8% D:0.9% F:29.7% | S:92.3% D:1.3% F:2.3% |
| Masking repetitive regions and gene prediction | ||||||||||
| Percentage masked bases (%) | 49.98 | — | — | 25.00 | 51.03 | 59.07 | 57.32 | 52.83 | 36.29 | 50.86 |
| Number of mRNA | 46,138 | — | — | 49,149 | 41,065 | 40,544 | 48,314 | 44,382 | — | 37,681 |
| Protein coding genes (CDS) | 46,138 | — | — | 49,149 | 41,065 | 35,119 | 48,314 | 44,382 | — | 37,681 |
| Functional annotated genes | 34,137 | — | — | — | — | 31,584 | 35,649 | 32,089 | — | — |
| Total gene length (Gb) | 0.83 | — | — | — | — | 0.90 | 1.13 | 0.86 | — | — |
| Total BUSCO for the predicted proteins (%) | ||||||||||
| + Euk database | S:86.7% D:9.4% F:3.5% | — | — | — | — | S:81.2% D:9.4% F:3.9% | S:83.9% D:13.7% F:2.0% | S:88.2% D:8.6% F:2.7% | — | — |
| + Met database | S:85.7% D:11.7% F:2.0% | — | — | — | — | S:82.3% D:10.3% F:3.2% | S:84.7% D:14.0% F:0.8% | S:86.0% D:11.3% F:2.3% | — | — |
Note.—BUSCO scores are presented for the Eukaryota (Euk) and Metazoa (Met) databases, showing the percentages of Complete Single (S), Complete Duplicate (D) and Fragmented (F) hits. mRNA, messenger ribonucleic acid.