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. Author manuscript; available in PMC: 2023 Jul 8.
Published in final edited form as: Biomater Sci. 2023 Apr 11;11(8):2886–2897. doi: 10.1039/d2bm02058k

Fig. 4.

Fig. 4

(A) Representative images of fibroblast global DNA methylation as indicated by 5-mC (green) staining and nuclei (DAPI) on glass and hydrogels following culture for 1 day. Inset images represent pixelated edges within the nuclei, used to quantify chromatin condensation percentage (CCP). Scale bars: 10 μm. Nuclear metrics were measured by (B) global DNA methylation intensity within the nucleus (C) the CCP, (D) nuclear spread area, and (E) nuclear shape index, which measures nuclear roundedness. N = 3 hydrogels per group. ****P < 0.0001, ***P < 0.001, *P < 0.05.