| Antibodies |
| HA |
Cell Signaling |
2367S; RRID:AB_10691311 |
| TOM40 |
Proteintech |
18409-1-AP; RRID:AB_2303725 |
| TIM50 |
Santa Cruz |
sc-393678; RRID:AB_2714191 |
| TIM23 |
BD Biosciences |
611222; RRID:AB_398754 |
| HSP90 |
Santa Cruz |
sc-7947; RRID:AB_2121235 |
| HSP90 |
Cell Signaling |
4874S; RRID:AB_2121214 |
| PINK1 |
Cell Signaling |
6946S; RRID:AB_11179069 |
| p62 |
Abcam |
ab56416; RRID:AB_945626 |
| LONP1 |
Proteintech |
66043-1-lg; RRID:AB_2881494 |
| LONP1 |
Novus Biologicals |
NBP1-81734; RRID:AB_11002818 |
| αTubulin |
Sigma |
T6199; RRID:AB_477583 |
| IRP2 |
Cell Signaling |
37135S; RRID:AB_2799110 |
| ALAS1 |
Abcam |
ab154860 |
| HMOX1 |
GeneTex |
GTX101147; RRID:AB_1950502 |
| OMA1 |
Santa Cruz |
sc-515788; RRID:AB_2905488 |
| TOM20 |
Santa Cruz |
sc-17764; RRID:AB_628381 |
| MPPα |
Sigma |
HPA021648; RRID:AB_1855491 |
| MPPβ |
Proteintech |
16064-1-AP; RRID:AB_2167122 |
| M2 |
Sigma |
F1804; RRID: AB_262044 |
| ATF4 |
Cell Signaling |
11815S; RRID:AB_2616025 |
| phospho-eif2α |
Cell Signaling |
3398; RRID:AB_2096481 |
| total-eif2α |
Cell Signaling |
5324; RRID:AB_10692650 |
| LIAS |
Proteintech |
11577-1-AP; RRID:AB_2135972 |
| SDHB |
Proteintech |
10620-1-AP; RRID:AB_2285522 |
| PPAT |
Proteintech |
15401-1-AP; RRID:AB_2166532 |
| FTMT |
Abcam |
ab66111 |
| OPA1 |
BD Biosciences |
612607; RRID:AB_399889 |
| PGAM5 |
Thermo Fisher Scientific |
PA5-57894; RRID:AB_2645430 |
| HRI |
Proteintech |
20499-1-AP; RRID:AB_10697665 |
| ISCU |
Proteintech |
14812-1-AP; RRID:AB_2280362 |
| NCL |
Cell Signaling |
14574; RRIS:AB_2798519 |
| TRAP1 |
Cell Signaling |
92345: RRID:AB_2800183 |
| Bacterial and virus strains |
| N/A |
|
|
| Biological samples |
|
|
| N/A |
|
|
| Chemicals, peptides, and recombinant proteins |
| Cycloheximide (CHX) |
Cayman Chemical |
14126 |
| MG132 |
Sigma |
C2211 |
| Bafilomycin A1 (Baf. A1) |
Cayman Chemical |
11038 |
| Deferoxamine (mesylate) (DFO) |
Cayman Chemical |
14595 |
| Deferiprone (DFP) |
Cayman Chemical |
20387 |
| CCCP |
Sigma |
C2759 |
| Hemin chloride |
Cayman Chemical |
16487 |
| Succinyl acetone (SA) |
MedChemExpress |
HY-W010184 |
| Proteinase K |
Sigma |
P6556 |
| Ferric ammonium citrate (FAC) |
Sigma |
RES20400-A7 |
| Erastin |
Cayman Chemical |
17754 |
| trans-ISRIB |
Cayman Chemical |
16258 |
| Doxycycline |
Sigma |
D9891 |
| DSP (dithiobis(succinimidyl propionate)) |
Thermo Fisher Scientific |
22585 |
| X-tremeGENE 9 DNA transfection reagent |
Roche |
6365787001 |
| Lipofectamine RNAi max transfection reagent |
Thermo Fisher Scientific |
13778075 |
| Tetramethylrhodamine, Methyl Ester, Perchlorate (TMRM) |
Thermo Fisher Scientific |
T668 |
| Critical commercial assays |
| Duolink In Situ PLA Probe Starter Kit |
Sigma |
DUO92101 |
| Deposited data |
| Raw images |
This study |
Mendeley Data (DOI: 10.17632/7p8w52928v.1) |
| RNA seq data |
This study |
SRA (accession number: PRJNA970328) |
| Experimental models: Cell lines |
| HEK293T |
ATCC |
CRL-3216 |
| HeLa |
ATCC |
CCL-2.2 |
| Endogenous DELE1-HA HEK293T |
Fessler et al., 2020
|
N/A |
| Tet-on DELE1-HA stable HeLa |
Houston et al., 2021
|
N/A |
| Tet-on Su9-DHFR-3xFlag stable HeLa |
Sekine et al., 2019
|
N/A |
| DELE1 KO HEK293T clone #24 |
This study |
N/A |
| DELE1 KO HEK293T clone #51 |
This study |
N/A |
| ATF4_uORF_mApple-stable HeLa |
This study |
N/A |
| Tet-on DELE1-HA / Mito-BFP stable HeLa |
This study |
N/A |
| MEL |
Millipore Sigma |
F4N |
| DELE1 KO MEL clone #3 |
This study |
N/A |
| DELE1 KO MEL clone #8 |
This study |
N/A |
| HRI KO MEL clone #1 |
This study |
N/A |
| Experimental models: Organisms/strains |
| N/A |
|
|
| Oligonucleotides |
| Stealth RNAi Negative Control Med GC Duplex #2 |
Thermo Fisher Scientific |
12935112 |
| Silencer Select Negative Control #1 siRNA |
Thermo Fisher Scientific |
4390843 |
| Stealth RNAi PARL |
Thermo Fisher Scientific |
HSS183080 |
| Stealth RNAi OMA1 |
Thermo Fisher Scientific |
HSS132976 |
| Stealth RNAi YME1L |
Thermo Fisher Scientific |
HSS116546 |
| Stealth RNAi HtrA2 |
Thermo Fisher Scientific |
HSS146986 |
| Stealth RNAi LONP |
Thermo Fisher Scientific |
HSS113887 |
| Silencer Select LONP #1 |
Thermo Fisher Scientific |
s17902 |
| Silencer Select LONP #2 |
Thermo Fisher Scientific |
s17903 |
| Stealth RNAi CLPP |
Thermo Fisher Scientific |
HSS112033 |
| Stealth RNAi AFG3L2 |
Thermo Fisher Scientific |
HSS116886 |
| Stealth RNAi SPG7 |
Thermo Fisher Scientific |
HSS110137 |
| Stealth RNAi MPPa |
Thermo Fisher Scientific |
HSS118357 |
| Stealth RNAi MPPb |
Thermo Fisher Scientific |
HSS114169 |
| Silencer Select HRI #1 |
Thermo Fisher Scientific |
s25822 |
| Silencer Select HRI #2 |
Thermo Fisher Scientific |
s25823 |
| Silencer Select DELE1 #1 |
Thermo Fisher Scientific |
s18966 |
| Silencer Select DELE1 #2 |
Thermo Fisher Scientific |
s18967 |
| Silencer Select ABCB7 #1 |
Thermo Fisher Scientific |
s855 |
| Silencer Select ABCB7 #2 |
Thermo Fisher Scientific |
s857 |
| Silencer Select ABCB8 |
Thermo Fisher Scientific |
s22104 |
| Silencer Select ABCB10 |
Thermo Fisher Scientific |
s23857 |
| Silencer Select ISCU #1 |
Thermo Fisher Scientific |
s23909 |
| Silencer Select ISCU #2 |
Thermo Fisher Scientific |
s23908 |
| Recombinant DNA |
| pLVX-puro-DELE1-HA |
Houston et al., 2021
|
N/A |
| pLVX-puro-DELE1 (TEV30)-HA |
This study |
N/A |
| pcDNA3.1 (+) Su9-TEV protease-HA |
Sekine et al., 2019
|
N/A |
| pRetroX-Tight-Pur-DELE1-HA |
Houston et al., 2021
|
N/A |
| pRetroX-Tight-Pur-DELE1(d30) -HA |
This study |
N/A |
| pRetroX-Tight-Pur-DELE1(d101)-HA |
This study |
N/A |
| pRetroX-Tight-Pur-pSu9-DHFR-tev-3xFlag |
Sekine et al., 2019
|
N/A |
| pCMV (d1) PINK1 (Tag-) |
Okatsu et al., 2012
|
N/A |
| pLVX-puro-PINK1-HA |
This study |
N/A |
| lentiCRISPR v2-hygro-human DELE1 (Guide#1) |
This study |
N/A |
| lentiCRISPR v2-hygro-human DELE1 (Guide#2) |
This study |
N/A |
| pXG237 |
Guo et al., 2020
|
Addgene #141281 |
| pLVX-puro-DELE1(d246-272)-HA |
This study |
N/A |
| pLVX-puro-TOMM20(ss)-DELE1(d101)-HA |
This study |
N/A |
| pLVX-puro-TOMM20(ss)-DELE1(d200)-HA |
This study |
N/A |
| pLVX-puro-DELE1(d102-200)-HA |
This study |
N/A |
| pLVX-puro-DELE1(d200-220)-HA |
This study |
N/A |
| pLVX-puro-DELE1(d101)-HA |
This study |
N/A |
| pLVX-puro-DELE1(d101/d102-200)-HA |
This study |
N/A |
| pLVX-puro-DELE1(d101/dTPR1)-HA |
This study |
N/A |
| pLVX-puro-DELE1(d101/dTPR2)-HA |
This study |
N/A |
| pLVX-puro-DELE1(d101/dTPR3)-HA |
This study |
N/A |
| pLVX-puro-DELE1(d101/dTPR4)-HA |
This study |
N/A |
| pLVX-puro-DELE1(d101/dTPR5)-HA |
This study |
N/A |
| pLVX-puro-DELE1(d102-150)-HA |
This study |
N/A |
| pLVX-puro-DELE1(d150-200)-HA |
This study |
N/A |
| pLVX-puro-DELE1(d102-200 + linker 20)-HA |
This study |
N/A |
| pLVX-puro-DELE1(d102-200 + linker 50)-HA |
This study |
N/A |
| pLVX-puro-DELE1(d102-200 + linker 100)-HA |
This study |
N/A |
| lentiCRISPR v2-puro-mouse DELE1(Guide#1) |
This study |
N/A |
| lentiCRISPR v2-puro-mouse DELE1 (Guide#2) |
This study |
N/A |
| lentiCRISPR v2-puro-mouse HRI (Guide#1) |
This study |
N/A |
| lentiCRISPR v2-puro-mouse HRI (Guide#2) |
This study |
N/A |
| Mito-BFP |
Friedman et al., 2011
|
Addgene #49151 |
| pLVX-puro-DELE1(d80-106)-HA |
This study |
N/A |
| pLVX-puro-PINK1(MTS)-DELE1(d101)-HA |
This study |
N/A |
| pLVX-puro-DELE1(dTPR1-7)-HA |
This study |
N/A |
| pLVX-puro |
Clontech |
632164 |
| pRetroX-Tight-Pur |
Clontech |
632104 |
| pRetroX-Tet-On Advanced |
Clontech |
632104 |
| lentiCRISPR v2-puro |
Sanjana et al., 2014
|
Addgene #52961 |
| lentiCRISPR v2-hygro |
Stringer et al., 2019
|
Addgene #98291 |
| Software and algorithms |
| BioRender |
N/A |
https://app.biorender.com/user/signin
|
| Other |
| N/A |
|
|
