Fig. 6.

HO-1 mediates ferroptosis by inhibiting the NF-κB signaling pathway in vivo and in vitro. (A) Western blot analysis of NF-κB, IKBα, and IKKβ in liver tissue. Values are the mean ± SD (n = 6 per group). *P < 0.05, **P < 0.01 compared to the WT ND group; #P < 0.05, ##P < 0.05 compared to the WT HFD group. (B, C) Western blot analysis of NF-κB, IKBα, and IKKβ in AML12 and HepG2 cells. Values are the mean ± SD. *P < 0.05, **P < 0.01 compared with the control group; #P < 0.05, ##P < 0.01 compared with the FFA group