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Validation of the expression of HO-1, ferroptosis-related key factors, and the NF-κB pathway in patient livers. (A) Representative H&E and Masson staining (×200 magnification) from healthy controls (n = 6) and NASH patients (n = 21). (A, B) Expression of HO-1, iron, GPX4, ACSL4, NF-κB, and IKKβ proteins was determined by immunohistochemistry staining (×400 magnification) in human liver tissues. The results are the mean ± SD. *P < 0.05, **P < 0.01 compared with the control group
