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Cytotoxicity effect of free lactoferrin on MDA-MB 231 cell line. The cytotoxicity effects of different concentrations of LF on MDA-MB 231 cell lines were evaluated by MTT assay after 24 h. The lowest cytotoxicity effects of LF on MDA-MB 231 cells occur at 10 mg/ml concentration and reach the maximum at 100 mg/ml concentration. ns: not significant, *: P ≤ 0.01, **: P ≤ 0.001, ***: P ≤ 0.0001
