Table 3.

Robustness expressed in % mean recovery and % relative standard deviation for GEM and BET

Parameter	Mean of area	% Mean recovery	Standard deviation	%RSD
GEM
Mobile phase (75:25)	1,47,425	99.61	0.015	0.252
Mobile phase (80:20)	1,48,821	100.55	0.053	0.910
Mobile phase (85:15)	1,47,800	99.86	0.025	0.427
Flow rate (0.9 ml/min)	1,49,147	100.77	0.062	1.064
Flow rate (1.0 ml/min)	1,50,343	100.99	0.071	1.219
Flow rate (1.1 ml/min)	1,47,460	99.64	0.016	0.268
Column Temp. (20 °C)	1,49,229	100.83	0.064	1.103
Column Temp. (25 °C)	1,49,715	100.59	0.055	0.942
Column Temp. (30 °C)	1,50,691	101.82	0.104	1.796
BET
Mobile phase (75:25)	61,101	101.35	0.058	1.040
Mobile phase (80:20)	60,583	100.48	0.023	0.421
Mobile phase (85:15)	60,767	100.79	0.036	0.644
Flow rate (0.9 ml/min)	61,266	99.51	0.014	0.259
Flow rate (1.0 ml/min)	62,144	100.93	0.041	0.745
Flow rate (1.1 ml/min)	61,599	100.05	0.007	0.121
Column Temp. (20 °C)	61,180	101.48	0.063	1.133
Column Temp. (25 °C)	63,467	100.95	0.042	0.757
Column Temp. (30 °C)	60,699	100.68	0.031	0.564