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. 2023 Jul 8;14(7):408. doi: 10.1038/s41419-023-05938-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Western blot analysis of UBE2S protein expression levels in UBE2S-silenced BCa cells. Representative images and quantitative analysis of wound healing (B), transwell migration (C) and invasion (D) assays in UBE2S-silenced BCa cells. E Representative image of the popliteal lymphatic metastasis model. Representative bioluminescence images (F) and quantitative analysis (G) of popliteal metastatic LNs from nude mice. LNs lymph nodes. Representative image of popliteal LNs (H) and quantitative analysis of their volumes (I). J Representative images of H&E staining in popliteal LNs from each group. Scale bars: black, 500 μm; blue, 50 μm. K Percentages of lymphatic metastasis in each group. *P < 0.05 and **P < 0.01.