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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Nat Methods. 2023 Jan 9;20(7):1082–1094. doi: 10.1038/s41592-022-01743-5

Figure 1. Photopick enables video-based pooled screening in mammalian cells.

Figure 1.

a. Photopick comprises a video-based pooled screening pipeline for enrichment of sensor variants with improved performance. b. Spectra of photo-taggable fluorescent proteins, mEos4a and PA-mCherry, used to validate the optical targeting system. Spectra from FPbase 61. c. Selective phototagging of mEos4a+ cells in a hybrid monolayer of mEos4a+, PAmCherry+ and non-fluorescent HEK cells. d. FACS analyses on the efficiency and fidelity of photoselection (Green channel: exc. 488 nm; Red channel: exc. 561 nm; n = 2 trials).