Figure 3.

¹H NMR spectra of the complex formed between TAR16s²U and the complementary TAR*16 RNA hairpin. (A) Imino spectra at 10°C of TAR*16 in 90% H₂O buffer, pH 6.4, containing 100 mM NaCl, 10 mM MgCl₂, 0.1 mM EDTA. A total of 128 transients were collected on a 0.5 mM sample. (B) Imino spectra at 10°C of TAR16s²U with buffer conditions as in (A). A total of 64 transients were collected on a 0.5 mM sample. (C) Imino spectra at 10°C of the complex formed between the two component hairpins in (A) and (B). A total of 512 transients were collected. The additional numbered peaks correspond to the imino proton resonances that result from base pairs at the kissing hairpin interface.