Figure 8.

Single Z-slices from confocal Z-stacks (A) are shown in (B) for each condition on Day 5 of culture. The single Z-slices show the cell networks across the field of view (20X magnification, scale bar = 100 μm). (C) The average intensity of the F-actin channel from (A) was averaged within 150 μm × 150 μm squares and can be represented as a heatmap of the image (D). (E) Heatmaps were taken from 30 successive steps in the Z-stacks across replicates, with the averages shown in (F). (G) The average of the three replicates is plotted across conditions. (H) Higher magnification examples show cells at the scaffold interface in a single Z-slice across conditions (scale bar = 10 μm).