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Protein–RNA binding and crosslinking. (A) Native mobility shift experiments of the wild-type PKR RBD and TFPAM-3-modified PKR RBD binding to a 92 nt RNA ligand. Lanes 1–10, 0, 0.005, 0.1, 0.8, 1.0, 2.0, 4.0, 6.0, 12.0 and 20.0 µM wild type or TFPAM-3-modified PKR RBD added, respectively. (B) Denaturing gel experiments resolving the PKR RBD/RNA ligand crosslinked complexes. Lanes 1–8, 0, 0.005, 0.1, 1.0, 2.0, 4.0, 6.0 and 12.0 µM TFPAM-3-modified PKR RBD added, respectively.
