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. 2000 May 1;28(9):1899–1905. doi: 10.1093/nar/28.9.1899

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Crosslinking of the PKR RBD to an RNA ligand. See Materials and Methods for photocrosslinking procedures. (A) Characterization of the crosslinked species. Lane 1, no protein added and no irradiation; lane 2, UV irradiation with no protein added; lane 3, 6 µM PKR RBD (E29C) with no UV irradiation; lane 4, PKR RBD (E29C) with 18 min UV irradiation; lane 5, TFPAM-3-modified PKR RBD (E29C) with no UV irradiation; lane 6, TFPAM-3-modified PKR RBD (E29C) with 18 min UV irradiation; lane 7, TFPAM-3-modified PKR RBD (E29C) with 18 min UV irradiation followed by proteinase K treatment. (B) Crosslinking as a function of the amino acid modified. All samples were UV irradiated for 18 min. Lane 1, no protein added; lane 2, PKR RBD (E13C); lane 3, TFPAM-modified PKR RBD (E13C); lane 4, PKR RBD (E29C); lane 5, TFPAM-modified PKR RBD (E29C); lane 6, PKR RBD (S33C); lane 7, TFPAM-modified PKR RBD (S33C).