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. 2023 Jul 9;11(13):e15688. doi: 10.14814/phy2.15688

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© 2023 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Differential shrinkage artifact and periglomerular/glomerular fibrosis in FSGS animals. (a) Bland–Altman plots show ratios between paraffin:plastic measurements of MGV obtained in each control animal from 10 or 20 glomeruli within respective techniques [11 comparisons from 6 control animals (green dots)]. (b) shows these same ratios of MGVs obtained from 10‐glomeruli in each FSGS animal [6 comparisons from 10 FSGS mice (red dots); X‐axis shows the corresponding MGVs]. (c) Violin plot (dashed line at median) summarizes the shrinkage artifact obtained from all measurements between FSGS and control animals [mean ± SEM; ****p < 0.0001]. (d) Photomicrographs (20×) from kidney sections of representative FSGS and control animals within our dataset showing variable glomerular fibrosis (area positive for Trichrome stain) in each.