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. Author manuscript; available in PMC: 2024 Jun 13.
Published in final edited form as: Immunity. 2023 Apr 13;56(6):1255–1268.e5. doi: 10.1016/j.immuni.2023.03.018

Figure 1: Neonates are resistant to systemic infection by Streptococcus pneumoniae, a ‘non-neonatal pathogen’.

Figure 1:

[A] Survival of WT neonatal (NNT, solid circle black line, n = 26), juvenile (JUV, half solid circle grey spotted line, n = 25) and adult mice (AD, open circle grey line, n = 18) following intraperitoneal (IP) challenge with P2406, an Spn strain with invasive potential in humans adapted in mice, at 5 x 101 – 1 x 102 colony forming units (CFU) per gram of body weight.

[B] Recoverable live Spn in blood of NNT and AD to assess systemic dissemination at two early time points, 24 hours (n = 16 for NNT, n = 11 for AD) and 32 hours (n = 19 for NNT, n = 13 for AD) post-infection, following IP challenge with Spn at 5 x 101 – 1 x 102 CFU per gram of body weight.

[C] Similar as described in [B], but for spleen at 24 hours (n = 16 for NNT, n = 11 for AD) and 32 hours post-infection (n = 17 for NNT, n = 11 for AD); BW, body weight.

[D] Recoverable live Spn in blood of NNT (n = 10) and JUV (n = 13) to assess systemic dissemination at 32 hours post-infection, following IP challenge with Spn at 5 x 101 – 1 x 102 CFU per gram of neonatal body weight.

[E] Similar as described in [D] but for spleen (n = 10 for NNT, n = 13 for JUV).

[F] Blood neutrophil counts from mice treated with an IgG isotype control clone 2A3 (n = 6) or neutrophil-specific depletion antibody clone 1A8 (n = 6) following IP challenge with Spn at 5 x 101 – 1 x 102 CFU per gram body weight at 32 hours post-infection.

[G] Recoverable live bacteria in spleen from mice described in [F].

[H] Blood monocyte counts from mice that were either wild-type, Ccr2+/+ (n = 15), or CCR2-deficient, Ccr2−/− (n = 9), following IP challenge with Spn at 5 x 101 – 1 x 102 CFU per gram body weight at 32 hours post-infection.

[I] Recoverable live bacteria in spleen from mice described in [H]. Each data point represents an individual mouse, and data are representative of experiments repeated at least twice. Data with error bars are presented as mean ± SEM. N.S., not significant.