Table 2:
Plasma and tissue biochemistry of male VECadCre+Et1.Cc1fl/fl double mutant mice and their double Flox and double Cre control littermates at 9 months of age
| VECadCre+Et1.Cc1+/+ | VECadCre-Et1.Cc1fi/fi | VECadCre+Et1.Cc1fi/fl | |
|---|---|---|---|
| Body weight (BW) (g) | 31.4 ± 0.3 | 30.1 ± 0.1 | 29.6 ± 0.5 |
| % WAT/BW | 2.3 ± 0.3 | 2.1 ± 0.4 | 2.2 ± 0.3 |
| Hepatic TG (μg/mg protein) | 57.2 ± 3.1 | 59.4 ± 3.6 | 56.9 ± 2.8 |
| Plasma TG (mg/dl) | 71.0 ± 4.7 | 69.9 ± 4.1 | 70.2 ± 3.5 |
| Plasma endothelin1 (pg/ml) | 6.1 ± 0.2 | 5.5 ± 1.2 | Negl |
| Plasma MMP2 (ng/ml) | 826.9 ± 45.6 | 843.8 ± 59.9 | 757.8 ± 23.3 |
| Plasma MMP13 (pg/ml) | 234.4 ± 89.1 | 246.6 ± 41.7 | 305.9 ± 64.4 |
| Plasma TIMP1 (pg/ml) | 1.4 ± 0.2 | 1.7 ± 0.7 | 1.4 ± 0.1 |
| Plasma ALT (mU/ml) | 39.7 ± 7.8 | 39.9 ± 5.2 | 35.9 ± 3.5 |
| Plasma AST (mU/ml) | 140.3 ± 11.9 | 136.1 ± 13.5 | 144.1 ± 10.4 |
| Plasma PDGF-B (ng/ml) | 6.0 ± 0.5 | 5.6 ± 0.4 | 5.4 ± 0.3 |
| Plasma NO (μM) | 35.1 ± 1.5 | 34.5 ± 1.0 | 35.4 ± 0.9 |
| Hepatic NAD/NADH | 1.3 ± 0.3 | 1.1 ± 0.2 | 1.4 ± 0.4 |
| Plasma 8-Isoprostane (pg/ml) | 5.5 ± 0.4 | 5.2 ± 0.5 | 4.9 ± 0.3 |
| Plasma IL-6 (pg/ml) | 3.7 ± 0.9 | 5.5 ± 1.9 | 6.1 ± 1.8 |
| Plasma TNFα (pg/ml) | 9.6 ± 2.7 | 10.8 ± 3.3 | 9.9 ± 2.9 |
Male mice (9 months of age, n=5/genotype) were fasted from 5:00 p.m. until 11:00 a.m. the next morning before blood was drawn and tissues were excised and plasma was processed. White adipose tissue (WAT) was collected and calculated relative to body weight (BW) to determine visceral adiposity. Values are expressed as mean ± SEM.