Figure 7.

SIRT7 overexpression in the dentate gyrus improves neurogenesis and cognitive function of aged brains.

Comparison of 10-month-old mice that were given stereotaxic injections of control lentivirus or lentivirus expressing SIRT7 into the dentate gyrus.

A, Schematic illustration of the experimental timeline.

B, GFP expression in mice given stereotaxic injections of GFP lentivirus into the dentate gyrus. Scale bar: 100 μm (left) and 50 μm (right).

C, D, Immunohistochemistry staining (C) and quantification (D) of Sox2-positive cells in the dentate gyrus. Blue: DAPI. Red: Sox2. Scale bar:100 μm.

E, F, Immunohistochemistry staining (E) and quantification (F) of newly differentiated Dcx-positive neurons in the dentate gyrus. Blue: DAPI. Red: Dcx. Scale bar:50 μm. G, H, Immunohistochemistry staining (G) and quantification (H) of long-term BrdU-retaining cells in the dentate gyrus. Blue: DAPI. Green: BrdU. Scale bar:100 μm.

I, J, Morris Water Maze test. Data shown are percent of time spent in the target and non-target quadrants (I) and the number of platform crossings in the target and non-target quadrants (J) during probe testing for memory. T: target quadrant. NT: non-target quadrant.

Error bars represent SE. *: p<0.05. **: p < 0.01. ***: p < 0.001. ns: p>0.05. Student’s t test.