. Author manuscript; available in PMC: 2024 Jul 1.

Published in final edited form as: J Mol Cell Cardiol. 2023 Apr 18;180:1–9. doi: 10.1016/j.yjmcc.2023.04.005

Table 1. Intracellular Ca2+ measurements in intact atrial myocytes.

Ca2+ handling parameters of Fura-2 acetoxymethyl-ester loaded atrial myocytes paced at 3 Hz followed by caffeine (10 mM) application. n=45 cells for +/+ vehicle, 44 cells for +/+ ent-verticilide, 33 cells for +/− vehicle, and 45 cells for +/− ent-verticilide. All data displayed as mean ± SD and analyzed using ANOVA multiple comparisons with Bonferroni-adjusted P values. CaT – pacing-induced Ca2+ transient, Caff.T – caffeine-induced Ca2+ transient

	(A) Pitx2+/+ vehicle (n=45)	(B) Pitx2+/+ ent-vert (n=44)	(C) Pitx2+/− vehicle (n=33)	(D) Pitx2+/− ent-vert (n=45)	P values
	(A) Pitx2+/+ vehicle (n=45)	(B) Pitx2+/+ ent-vert (n=44)	(C) Pitx2+/− vehicle (n=33)	(D) Pitx2+/− ent-vert (n=45)	(A) vs (B)	(A) vs (C)	(C) vs (D)
Diastolic Ca2+ (F_Ratio)	0.60 ± 0.07	0.56 ± 0.06	0.60 ± 0.09	0.59 ± 0.05	0.10	>0.99	>0.99
Time to peak (ms)	30.79 ± 5.04	29.45 ± 3.07	29.17 ± 3.03	29.36 ± 4.12	0.78	0.54	>0.99
CaT decay rate constant (ms)	114.2 ± 23.0	123.6 ± 24.6	114.1 ± 14.7	114.9 ± 18.8	0.24	>0.99	>0.99
SR Ca2+ content (F_Ratio)	0.83 ± 0.17	0.78 ± 0.16	0.91 ± 0.15	0.86 ± 0.09	0.89	0.10	>0.99
Caff.T decay rate constant (s)	2.95 ± 0.27	2.85 ± 0.38	2.85 ± 0.32	2.99 ± 0.32	>0.99	>0.99	0.53