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. Author manuscript; available in PMC: 2024 Jun 23.
Published in final edited form as: Circ Res. 2023 May 8;133(1):e1–e16. doi: 10.1161/CIRCRESAHA.122.322213

Figure 6. FKBP5 negatively regulates HIF-1α.

Figure 6.

(A) Increased HIF-1α protein level in the atria of cKD mice revealed by Western blots. (B) Immunostaining of HIF-1α in the HEK 293 cells transfected with pcDNA.Gfp (control) or pcDNA.Fkbp5.Gfp. (C) Representative Western blots with the cytosol- or nuclear-fractions of H9C2 cells transfected with pcDNA (control) or pcDNA.Fkbp5 (FKBP5-OE) vectors. (D) Relative level of the nuclear HIF-1α protein normalized to Histone H3 in H9C2 cells. (E) Relative mRNA levels of Slc8a1 in H9C2 cells treated with pcDNA vector (control) or pcDNA.Fkbp5 (FKBP5-OE). (F) Co-immunoprecipitation of HSP90 (IP) and HIF-1α (Western blots) in H9C2 cells treated with pcDNA (control) or pcDNA.Fkbp5 (FKBP5-OE) vectors. (G) Representative Western blots and (H) quantification of HIF-1α protein levels in the pcDNA or pcDNA.Fkbp5 vector transfected H9C2 cells, in the presence of vehicle (Veh), 17-AAG (HSP90 inhibitor), or MG (MG-132, proteasome inhibitor). p-values were determined with Mann-Whitney test in A and D, Shapiro-Wilk test and the unpaired Student’s t-test in E, and two-way ANOVA with Tukey’s comparison in H.