Figure 4.

Knockdown of GCKR, SNX17, and HP alter antithrombin production in HepG2 cells. A) Antithrombin secreted into the culture supernatant was detected by immunoblot and quantified by densitometry. B) SERPINC1 expression and C) PROC expression were measured by RT-qPCR using the ΔΔCt method to compare mRNA abundance and 18S as the reference gene. Bars and error bars indicate mean and standard error of the mean; Numbers indicate biological replicates; Statistical comparisons were performed by one-way ANOVA and Šidák’s multiple comparisons tests.